Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation

Teng Wu; Min Tan; Heng-Ye Gong; Yong Wang; Xin-Tao Shuai

doi:10.1007/s10118-018-2158-z

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Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation

ARTICLE | Updated：2021-02-18

- Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation
- Chinese Journal of Polymer Science Vol. 36, Issue 12, Pages: 1312-1320(2018)
- Affiliations：
  
  a.PCFM Lab of Ministry of Education, School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China
  b.Center of Biomedical Engineering, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
- Author bio：
  
  shuaixt@mail.sysu.edu.cn
- Funds：
- DOI：10.1007/s10118-018-2158-z
  CLC：
- Received：25 April 2018，
  
  Revised：2018-5-9，
  
  Accepted：10 May 2018，
  
  Online First：22 June 2018，
  
  Published：2018-12
- Accepted：
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Citation: Wu, T.; Tan, M.; Gong, H. Y.; Wang, Y.; Shuai, X. T. Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation. Chinese J. Polym. Sci. 2018, 36(12), 1312–1320

Teng Wu, Min Tan, Heng-Ye Gong, et al. Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation[J]. Chinese Journal of Polymer Science, 2018, 36(12): 1312-1320.
Citation: Wu, T.; Tan, M.; Gong, H. Y.; Wang, Y.; Shuai, X. T. Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation. Chinese J. Polym. Sci. 2018, 36(12), 1312–1320 DOI： 10.1007/s10118-018-2158-z.

Teng Wu, Min Tan, Heng-Ye Gong, et al. Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation[J]. Chinese Journal of Polymer Science, 2018, 36(12): 1312-1320. DOI： 10.1007/s10118-018-2158-z.

摘要

Abstract

Chronic inflammatory responses induced by macrophages play a pivotal role in the progression of atherosclerosis. In the present study

a multifunctional nanocarrier based on poly(ethylene glycol)-block-poly(L-aspartic acid) grafted with diethylenetriamine

lysine and cholic acid (PEG-PAsp(DETA)-Lys-CA

) polymer was synthesized for co-delivery of andrographolide and siRNA targeting Notch1 gene to alleviate the inflammatory response in macrophages. The nanocarrier exerted low cytotoxicity as well as high performance in drug/siRNA co-delivery.

In vitro

studies demonstrated the co-delivery of andrographolide and Notch1 siRNA not only significantly inhibited lipopolysaccharide (LPS)-activated interleukin-6 (IL-6) and monocytes chemotactic protein 1 (MCP-1) expression as well as blocked nuclear factor-κB (NF-κB) signal activation

but also interfered the Notch1 gene expression and increased anti-inflammatory cytokines such as interleukin-10 (IL-10) and arginase-1 expression obviously in macrophages. These results suggested that the combination therapy based on Notch1 siRNA and andrographolide co-delivered nanocarrier

i.e.

suppressing the expression of pro-inflammatory cytokines while simultaneously increasing anti-inflammatory factors expression

be a feasible strategy for atherosclerosis treatment.

关键词

Keywords

references

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