Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush

Jie Zhao; Ru Mo; Li-Mei Tian; Ling-Jie Song; Shi-Fang Luan; Jing-Hua Yin; Lu-Quan Ren

doi:10.1007/s10118-018-2031-0

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Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush

Articles | Updated：2021-02-18

- Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush
- Chinese Journal of Polymer Science Vol. 36, Issue 4, Pages: 472-478(2018)
- Affiliations：
  
  a.Key Laboratory of Bionic Engineering, Ministry of Education, Jilin University, Changchun 130022, China
  b.State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  c.Jilin Province People's Hospital, Changchun 130021, China
- Author bio：
  
  Jie Zhao, E-mail jiezhao@jlu.edu.cn
- Funds：
- DOI：10.1007/s10118-018-2031-0
  CLC：
- Received：23 August 2017，
  
  Accepted：30 August 2017，
  
  Online First：01 December 2017，
  
  Published：05 April 2018
- Accepted：
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Jie Zhao, Ru Mo, Li-Mei Tian, et al. Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush[J]. Chinese Journal of Polymer Science, 2018, 36(4): 472-478.
DOI：

Jie Zhao, Ru Mo, Li-Mei Tian, et al. Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush[J]. Chinese Journal of Polymer Science, 2018, 36(4): 472-478. DOI： 10.1007/s10118-018-2031-0.

摘要

Abstract

High sensitive immunoassay platforms have gained intense attention due to their vital roles in early-stage disease diagnosis and therapeutic information feedback. Although random covalent-binding of antibody has been widely adopted in immunoassays due to its simplicity and effectiveness

it readily loses its activity and fails to exhibit high antigen-binding capacity. In this work

copolymer of zwitterionic sulfobetaine methacrylate (SBMA) and glycidyl methacrylate (GMA) brushes were immobilized onto inert polypropylene (PP)

via

surface-initiated atom transfer radical polymerization (ATRP) based on biomimetic dopamine pretreatment. Subsequently

boronic acid (BA) groups were covalently bonded

via

active GMA units

followed by the introduction of oriented immobilization of antibody. Owing to the oriented immobilization of antibody facilitated by BA groups in polymer brush

the bioactivity of antibody is well preserved

which endows the surface with significantly enhanced antigen-binding capacity. Moreover

the existence of SBMA segments in polymer brushes renders the surface high resistance to nonspecific protein adsorption

significantly alleviating the signal interference of antigen recognition. This strategy could find potential applications in developing high sensitive immunoassay platforms based on the different substrates.

关键词

Keywords

references

Y. M. Wu , Y. Cen , L.J. Huang , R. Q. Yu , X. Chu . Upconversion fluorescence resonance energy transfer biosensor for sensitive detection of human immunodeficiency virus antibodies in human serum . Chem. Commun. , 2014 . 50 4759 - 4762 . DOI: 10.1039/C4CC00569D http://doi.org/10.1039/C4CC00569D .

X. Bi , Z. Liu . Facile preparation of glycoprotein-imprinted 96-well microplates for enzyme-linked immunosorbent assay by boronate affinity-based oriented surface imprinting . Anal. Chem. , 2014 . 86 ( 1 ): 959 - 966 . DOI: 10.1021/ac403736y http://doi.org/10.1021/ac403736y .

S. D. Gan , K. R. Patel . Enzyme immunoassay and enzyme-linked immunosorbent assay . J. Invest. Dermatol. , 2013 . 133 ( 9 ): 1 - 3 . http://www.sciencedirect.com/science/article/pii/S0022202X15363879 http://www.sciencedirect.com/science/article/pii/S0022202X15363879 , .

Y. M. Bae , B. K. Oh , W. Lee , W. H. Lee , J. W. Choi . Study on orientation of immunoglobulin G on protein G layer . Biosens. Bioelectron. , 2005 . 21 ( 1 ): 103 - 110 . DOI: 10.1016/j.bios.2004.09.003 http://doi.org/10.1016/j.bios.2004.09.003 .

P. Bhadra , M. S. Shajahan , E. Bhattacharya , A. Chadha . Studies on varying n -alkanethiol chain lengths on a gold coated surface and their effect on antibody-antigen binding efficiency . RSC Adv. , 2015 . 5 ( 98 ): 80480 - 80487 . DOI: 10.1039/C5RA11725A http://doi.org/10.1039/C5RA11725A .

Y. Jung , J.Y. Jeong , B.H. Chung . Recent advances in immobilization methods of antibodies on solid supports . Analyst , 2008 . 133 ( 6 ): 697 - 701 . DOI: 10.1039/b800014j http://doi.org/10.1039/b800014j .

M. B. Soellner , K. A. Dickson , B. L. Nilsson , R.T. Raines . Site-specific protein immobilization by staudinger ligation . J. Am. Chem. Soc. , 2003 . 125 ( 39 ): 11790 - 11791 . DOI: 10.1021/ja036712h http://doi.org/10.1021/ja036712h .

A. K. Trilling , J. Beekwilder , H. Zuilhof . Antibody orientation on biosensor surfaces:a minireview . Analyst , 2013 . 138 ( 6 ): 1619 - 1627 . DOI: 10.1039/c2an36787d http://doi.org/10.1039/c2an36787d .

Z. Wang , G. J. Jin . Feasibility of protein A for the oriented immobilization of immunoglobulin on silicon surface for a biosensor with imaging ellipsometry . Biochem. Biophys. Methods , 2003 . 57 ( 3 ): 203 - 211 . DOI: 10.1016/S0165-022X(03)00109-X http://doi.org/10.1016/S0165-022X(03)00109-X .

J. Kim , J. Cho , P. M. Seidler , N. E. Kurland , V. K. Yadavalli . Investigations of chemical modifications of amino-terminated organic films on silicon substrates and controlled protein immobilization . Langmuir, , 2010 . 26 ( 4 ): 2599 - 2608 . DOI: 10.1021/la904027p http://doi.org/10.1021/la904027p .

E. de Juan-Franco , A. Caruz , J. R. Pedrajas , L. M. Lechuga . Site-directed antibody immobilization using a protein A-gold binding domain fusion protein for enhanced SPR immunosensing . Analyst , 2013 . 138 ( 7 ): 2023 - 2031 . DOI: 10.1039/c3an36498d http://doi.org/10.1039/c3an36498d .

S. Wang , J. Ye , X. Li , Z. Liu . Boronate affinity fluorescent nanoparticles for förster resonance energy transfer inhibition assay of cis -diol biomolecules . Anal. Chem. , 2016 . 88 ( 10 ): 5088 - 5096 . DOI: 10.1021/acs.analchem.5b04507 http://doi.org/10.1021/acs.analchem.5b04507 .

J. A. Peters . Interactions between boric acid derivatives and saccharides in aqueous media:structures and stabilities of resulting esters . Coord. Chem. Rev. , 2014 . 268 ( 2 ): 1 - 22 . https://www.sciencedirect.com/science/article/pii/S0010854514000204 https://www.sciencedirect.com/science/article/pii/S0010854514000204 , .

W. L. Brooks , B. S. Sumerlin . Synthesis and applications of boronic acid-containing polymers:from materials to medicine . Chem. Rev. , 2016 . 116 ( 3 ): 1375 - 1397 . DOI: 10.1021/acs.chemrev.5b00300 http://doi.org/10.1021/acs.chemrev.5b00300 .

M. L. Chen , A. K. Adak , N. C. Yeh . Fabrication of an oriented Fc-fused lectin microarray through boronate formation . Angew. Chem. , 2008 . 47 ( 45 ): 8627 - 8630 . DOI: 10.1002/anie.v47:45 http://doi.org/10.1002/anie.v47:45 .

L. Tan , J. Xing , F. Cao , L. Chen , C. Zhang , R. Shi , Y. Wang . Synthesis of double-hydrophilic double-grafted copolymers PMA- g -PEG/PDMA and their protein-resistant properties . Chinese J. Polym. Sci. , 2013 . 31 ( 4 ): 691 - 701 . DOI: 10.1007/s10118-013-1254-3 http://doi.org/10.1007/s10118-013-1254-3 .

S. Luan , J. Zhao , H. Yang , H. Shi , J. Jin , X. Li , J. Liu , J. Wang , J. Yin , P. Stagnaro . Surface modification of poly(styrene- b -(ethylene- co -butylene)- b -styrene) elastomer via UV-induced graft polymerization of N -vinyl pyrrolidone . J. Colloid Interf. Sci. , 2012 . 93 ( 1 ): 127 - 134 . http://www.ncbi.nlm.nih.gov/pubmed/22264686 http://www.ncbi.nlm.nih.gov/pubmed/22264686 , .

C. J. Huang , Y. Li , J. B. Krause , N. D. Brault , S. Jiang . Internal architecture of zwitterionic polymer brushes regulates nonfouling properties . Macromol. Rapid Commun. , 2012 . 33 1003 - 1007 . DOI: 10.1002/marc.201100858 http://doi.org/10.1002/marc.201100858 .

H. Chien , C. Tsai , W. Tsai , M. J. Wang , W. H. Kuo , T. C. Wei , S. T. Huang . Surface conjugation of zwitterionic polymers to inhibit cell adhesion and protein adsorption . J. Colloid Interf. Sci. , 2013 . 107 ( 7 ): 152 - 159 . https://www.sciencedirect.com/science/article/pii/S0927776513000945 https://www.sciencedirect.com/science/article/pii/S0927776513000945 , .

Q. Yu , Y. Zhang , H. Wang , J. Brash , H. Chen . Anti-fouling bioactive surfaces . Acta Biomater. , 2011 . 7 ( 4 ): 1550 - 1557 . DOI: 10.1016/j.actbio.2010.12.021 http://doi.org/10.1016/j.actbio.2010.12.021 .

M. Zong , Y. Gong . Fabrication and biocompatibility of cell outer membrane mimetic surfaces . Chinese J. Polym. Sci. , 2011 . 29 ( 1 ): 53 - 64 . DOI: 10.1007/s10118-010-1019-1 http://doi.org/10.1007/s10118-010-1019-1 .

J. Zhao , Q. Shi , S. Luan , L. Song . Polypropylene non-woven fabric membrane via surface modification with biomimetic phosphorylcholine in Ce(Ⅳ)/HNO 3 redox system . Mater. Sci. Eng. C , 2012 . 32 ( 7 ): 1785 - 1789 . DOI: 10.1016/j.msec.2012.04.057 http://doi.org/10.1016/j.msec.2012.04.057 .

Y. Li , M. Zhou , C. Geng , F. Chen , Q. Fu . Simultaneous improvements of thermal stability and mechanical properties of poly(propylene carbonate) via incorporation of environmental-friendly polydopamine . Chinese J. Polym. Sci. , 2014 . 32 ( 12 ): 1724 - 1736 . DOI: 10.1007/s10118-014-1518-6 http://doi.org/10.1007/s10118-014-1518-6 .

M. E. Lynge , R. van der Westen , A. Postma , B. Stadler . Polydopamine-a nature-inspired polymer coating for biomedical science . Nanoscale , 2011 . 3 ( 12 ): 4916 - 4928 . DOI: 10.1039/c1nr10969c http://doi.org/10.1039/c1nr10969c .

J. H. Jiang , L. P. Zhu , X. L. Li , Y. Y. Xu , B. K. Zhu . Surface modification of PE porous membranes based on the strong adhesion of polydopamine and covalent immobilization of heparin . J. Membr. Sci. , 2010 . 364 ( 1-2 ): 194 - 202 . DOI: 10.1016/j.memsci.2010.08.017 http://doi.org/10.1016/j.memsci.2010.08.017 .

X. Zhong , H. J. Bai , J. J. Xu , H. Y. Chen , Y. H. Zhu . A reusable interface constructed by 3-aminophenylboronic acid-functionalized multiwalled carbon nanotubes for cell capture, release, and cytosensing . Adv. Funct. Mater. , 2010 . 20 ( 6 ): 992 - 999 . DOI: 10.1002/adfm.200901915 http://doi.org/10.1002/adfm.200901915 .

Y. Jung , J. M. Lee , H. Jung , B. H. Chung . Self-directed and self-oriented immobilization of antibody by protein G-DNA conjugate . Anal. Chem. , 2007 . 79 ( 17 ): 6534 - 6541 . DOI: 10.1021/ac070484i http://doi.org/10.1021/ac070484i .

D. Li , Y. Chen , Z. Liu . Boronate affinity materials for separation and molecular recognition:structure, properties and applications . Chem. Soc. Rev. , 2015 . 44 ( 22 ): 8097 - 8123 . DOI: 10.1039/C5CS00013K http://doi.org/10.1039/C5CS00013K .

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