Polyglutamic Acid Based Polyanionic Shielding System for Polycationic Gene Carriers

Jia-liang Xia; Hua-yu Tian; Jie Chen; Zhao-pei Guo; Lin Lin; Hong-yan Yang; Zong-cai Feng

doi:10.1007/s10118-016-1756-x

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Polyglutamic Acid Based Polyanionic Shielding System for Polycationic Gene Carriers

Updated：2021-06-21

- Polyglutamic Acid Based Polyanionic Shielding System for Polycationic Gene Carriers
- Chinese Journal of Polymer Science Vol. 34, Issue 3, Pages: 316-323(2016)
- Affiliations：
  
  a.School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang 524048, China
  b.Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China
  c.Research Center of Chemistry & Materials, Lingnan Normal University, Development Center for New Materials Engineering & Technology in Universities of Guangdong, Zhanjiang 514048, China
- Author bio：
  
  **Hua-yu Tian (田华雨), E-mail: thy@ciac.ac.cn
- Funds：
- DOI：10.1007/s10118-016-1756-x
  CLC：
- Received：02 September 2015，
  
  Revised：2015-11-08，
  
  Accepted：12 November 2015，
  
  Published：2016-03
- Accepted：
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Jia-liang Xia, Hua-yu Tian, Jie Chen, et al. Polyglutamic Acid Based Polyanionic Shielding System for Polycationic Gene Carriers[J]. Chinese Journal of Polymer Science, 2016, 34(3): 316-323.
DOI：

Jia-liang Xia, Hua-yu Tian, Jie Chen, et al. Polyglutamic Acid Based Polyanionic Shielding System for Polycationic Gene Carriers[J]. Chinese Journal of Polymer Science, 2016, 34(3): 316-323. DOI： 10.1007/s10118-016-1756-x.

摘要

Abstract

For the purpose of increasing the

in vivo

stability of polycation gene carriers

we prepared a kind of pH-sensitive poly(ethylene glycol)-poly (

-benzyl-L-glutamate-co-glutamic acid) (PEG-PGA(65)

65 denotes the molar ratio of glutamic acid in poly(

-benzyl-L-glutamate-co-glutamic acid)). PEG-PGA(65) showed low cytotoxicity and could shield the positive charge of DNA/PEI (1:1) polyplexes efficiently. The transfection was enhanced due to the partially charge shielding in HeLa cell line at pH of 7.4. There was almost no transfection efficiency when the surface charge of the ternary particles turned to negative at pH of 7.4. However

the transfection efficiency recovered a lot by culturing at pH of 6.0 at the beginning of transfection. Confocal microscopic observation and flow cytometry results showed DNA/PEI polyplexes should be efficiently released and endocytosized at pH 6.0

because of the pH triggered deshielding action of PEG-PGA(65). Due to the good biocompatibility and suitable pH triggered shielding/deshielding property

PEG-PGA(65) could be a potential shielding system for polycationic gene carriers used

in vivo

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