Effects of Chirality on Gene Delivery Efficiency of Polylysine

Bin-wei Zhao; Zhuxian Zhou; Youqing Shen

doi:10.1007/s10118-016-1735-2

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Effects of Chirality on Gene Delivery Efficiency of Polylysine

Updated：2021-06-21

- Effects of Chirality on Gene Delivery Efficiency of Polylysine
- Chinese Journal of Polymer Science Vol. 34, Issue 1, Pages: 94-103(2016)
- Affiliations：
  
  Center for Bionanoengineering and State Key Laboratory for Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China
- Author bio：
  
  **Youqing Shen (申有青), E-mail: Shenyq@zju.edu.cn
- Funds：
- DOI：10.1007/s10118-016-1735-2
  CLC：
- Received：13 July 2015，
  
  Revised：2015-08-17，
  
  Accepted：04 September 2015，
  
  Published：2016-01
- Accepted：
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Bin-wei Zhao, Zhuxian Zhou, Youqing Shen. Effects of Chirality on Gene Delivery Efficiency of Polylysine[J]. Chinese Journal of Polymer Science, 2016, 34(1): 94-103.
DOI：

Bin-wei Zhao, Zhuxian Zhou, Youqing Shen. Effects of Chirality on Gene Delivery Efficiency of Polylysine[J]. Chinese Journal of Polymer Science, 2016, 34(1): 94-103. DOI： 10.1007/s10118-016-1735-2.

摘要

Abstract

Chirality is a key factor in the biological activity of many biomolecules. Poly(L-lysine) (PLL)

a polypeptide synthesized from L-lysine

is one of the mostly used cationic polymers for gene delivery. The effect of chirality of polylysine (PL) on its gene delivery remains unknown. Herein

we prepared three polylysines (PLs) with the similar molecular weight but different backbone chiralities including poly(L-lysine) (PLL)

poly(D-lysine) (PDL) and poly(DL-lysine) (PDLL). The side chains of each PL were modified with propylene oxide (PO) of different chiralities including (R)PO

(S)PO and (R

S)PO. These PL-POs with distinct chirality in main and side chains could condense pDNA into polyplexes. The polyplexes had approximately the same size

zeta potential and binding ability

but showed distinct gene transfection efficiency. We found that the PLs of L-configuration in the main chain had higher transfection efficiency than that of D or DL configuration due to their faster cellular uptake

while the side chain chirality had no effect on transfection efficiency.

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