A series of cellulose 3

5-dimethylphenylcarbamates (CDMPCs) with different degrees of substitution (DS) and degrees of polymerization (DP) were homogeneously synthesized in 1-allyl-3-methylimidazolium chloride (AmimCl). Then

the CDMPCs were coated on silica gel and used as chiral stationary phases (CSPs)

and their chiral recognition abilities for seven racemates were evaluated by high performance liquid chromatography. The results showed that DS and DP of CDMPCs had a great influence on chiral recognition abilities of the CSPs. The CSPs with the DS ≈ 1 gives a low chiral recognition to most racemates. On the contrast

the CSPs with the DS ≥ 2 exhibited high chiral separation abilities. For example

six racemates could be separated on the CSP with CDMPC of DS ≈ 2 (CSP-2). Especially

for the enantioseparation of 1-(2-naphthyl) ethanol and Tröger's base

CSP-2 gave the highest separation ability in all of CSPs. On the other hand

when the DP of cellulose was in a range from 39 to 220

the chiral separation abilities of CDMPCs increased as the DP increased. This work demonstrates that the structure of cellulose esters such as DS and DP has important effect on their chiral separation ability

and therefore provides a practical method to design and prepare desirable CSPs for different racemates.