Guanidinoamidized Linear Polyethyleneimine for Gene Delivery

Bo Zhang; Xin-peng Ma; Mei-hua Sui; Edward Van Kirk; William J. Murdoch; Maciej Radosz; Neng-ming Lin; You-qing Shen

doi:10.1007/s10118-015-1644-9

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Guanidinoamidized Linear Polyethyleneimine for Gene Delivery

PAPER | Updated：2021-06-21

- Guanidinoamidized Linear Polyethyleneimine for Gene Delivery
- Chinese Journal of Polymer Science Vol. 33, Issue 6, Pages: 908-919(2015)
- Affiliations：
  
  a.Institute for Individualized Medicine, Hangzhou First People’s Hospital, Hangzhou 310006, China
  b.Center for Bionanoengineering and the State Key Laboratory for Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China
  c.Laboratory of Clinical Pharmacy, Zhejiang Cancer Hospital, Hangzhou 310022, China
  d.Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming 82071, USA
  e.Department of Animal Science, University of Wyoming, Laramie, Wyoming 82071, USA
- Author bio：
  
  † These two authors are joint first authors.
  **You-qing Shen (申有青), E-mail: shenyq@zju.edu.cn
  Neng-ming Lin (林能明), E-mail: lnm1013@163.com
- Funds：
- DOI：10.1007/s10118-015-1644-9
  CLC：
- Received：22 October 2014，
  
  Revised：2014-11-12，
  
  Accepted：27 November 2014，
  
  Published：2015-06
- Accepted：
Scan QR Code
Bo Zhang, Xin-peng Ma, Mei-hua Sui, et al. Guanidinoamidized Linear Polyethyleneimine for Gene Delivery[J]. Chinese Journal of Polymer Science, 2015, 33(6): 908-919.
DOI：

Bo Zhang, Xin-peng Ma, Mei-hua Sui, et al. Guanidinoamidized Linear Polyethyleneimine for Gene Delivery[J]. Chinese Journal of Polymer Science, 2015, 33(6): 908-919. DOI： 10.1007/s10118-015-1644-9.

摘要

Abstract

Guanidine was introduced to low molecular weight linear polyethyleneimine (LPEI)

via

amide groups

to explore the effect of both guanidine degree and pendant chain length on its transfection behavior. The resulting guanidinoamidized LPEIs (GLPEIs) could dramatically reduce LPEI’s toxicity

enhance its DNA-packaging capability

cellular uptake and therefore transfection efficiency. These polyplexes were taken up very efficiently

via

caveolae-mediated endocytosis and their transfection efficiencies in ovarian cancer cells were significantly improved compared to native LPEI

10k

polyplexes. Among these GLPEIs

LPEI-C

-G

100

showed higher DNA affinity even than LPEI

25k

and the highest transfection efficiency

probably due to the optimization of polymer chain flexibility. Of notice

LPEI-C

-G

100

polyplexes could more effectively accumulate into cytoplasm than LPEI

25k

although the transfection efficiency of LPEI-C

-G

100

polyplexes was not superior to that of LPEI

25k

polyplexes

which would be probably attributed to the more efficient release of LPEI

25k

polyplexes than LPEI-C

-G

100

polyplexes in the cytoplasm.

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