Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution

Ji-han Zhou; Hao Wen; Cui-cui Su; Lin Niu; De-hai Liang

doi:10.1007/s10118-014-1478-x

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Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution

Updated：2021-06-21

- Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution
- Chinese Journal of Polymer Science Vol. 32, Issue 11, Pages: 1460-1468(2014)
- Affiliations：
  
  Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
- Author bio：
  
  **De-hai Liang (梁德海), Email: dliang@pku.edu.cn
- Funds：
- DOI：10.1007/s10118-014-1478-x
  CLC：
- Received：03 January 2014，
  
  Revised：2014-02-21，
  
  Accepted：25 February 2014，
  
  Published：2014-11
- Accepted：
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Ji-han Zhou, Hao Wen, Cui-cui Su, et al. Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution[J]. Chinese Journal of Polymer Science, 2014, 32(11): 1460-1468.
DOI：

Ji-han Zhou, Hao Wen, Cui-cui Su, et al. Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution[J]. Chinese Journal of Polymer Science, 2014, 32(11): 1460-1468. DOI： 10.1007/s10118-014-1478-x.

摘要

Abstract

Using three designed peptides with precisely-controlled charge density and three types of DNAs with different length and flexibility

the effect of charge density on the formation of PEC was studied. Highly charged (KKKK)

interacts strongly with 21 bp dsDNA to form large complex

followed by precipitation; while the medium charged (KGKG)

only form complex with 21 bp dsDNA at proper +/− charge ratios; and no prominent complex between weakly charged (KGGG)5 and 21 bp dsDNA is observed at the same conditions. Similar trend is observed when the peptides form complex with 2000 bp DNA or 21nt ssDNA. It is also found that the complex formed by adding peptide to DNA is in random coil conformation

but the complex prepared by the inverse order is in molten globule state. Re-dissolution of the complex occurs only when DNA is added to peptides with similar or shorter length.

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Related Institution

Key Laboratory for Neurodegenerative Diseases Nanomedicine of Hubei Province; School of Chemistry, Chemical Engineering and Life Science; Institute WUT-AMU, Wuhan University of Technology

Beijing National Laboratory for Molecular Sciences, Department of Polymer Science and Engineering and the Key Laboratory of Polymer Chemistry and Physics of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University

Beijing National Laboratory for Molecular Sciences and Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University

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