A new method to prepare polysaccharide-coating type chiral stationary phases (CSPs) was developed in this work. As a typical example

naked silica gel was coated by cellulose

which was then derivatized with 3

5-dimethylbenzoyl chloride to afford cellulose tris(3

5-dimethylbenzoate)-silica gel (CTDBS) complex. The silanols on CTDBS were end-capped with 3- aminopropyltriethoxysilane to obtain CSP

1

. The amino groups on CSP

1

were further end-capped with 3

5-dimethylbenzoyl chloride to give CSP

2

. The silanols on CTDBS were end-capped with methyltrimethoxysilane to yield CSP

3

. CSPs

1−3

were characterized by FTIR

solid-state

13

C-NMR and elemental analysis. The enantioseparation abilities of CSPs

1−3

were evaluated with structurally various chiral analytes. The enantioseparation results demonstrated that the end-capping moieties on CSPs

1

and

2

significantly affected enantioseparation. In addition

the effect of the structures of chiral analytes and endcapping moieties on the retention factors and the resolutions was discussed.