CONTRIBUTION OF CHOLESTEROL MOIETIES ATTACHED ON MPEG-<italic style="font-style: italic">b</italic>-PCL-<italic style="font-style: italic">b</italic>-PLL TO THE CELL UPTAKE, ENDOSOMAL ESCAPE AND GENE KNOCKDOWN OF THE MICELLEPLEXES OF siRNA

Ruo-gu Qi; Su-hong Wu; Yu Wang; Jie Chen; Zhi-gang Xie; Yu-bin Huang; Xia-bin Jing

doi:10.1007/s10118-013-1288-6

Your Location：

Home >

Browse articles >

CONTRIBUTION OF CHOLESTEROL MOIETIES ATTACHED ON MPEG-b-PCL-b-PLL TO THE CELL UPTAKE, ENDOSOMAL ESCAPE AND GENE KNOCKDOWN OF THE MICELLEPLEXES OF siRNA

Special Issue: In Honor of the 80th Birthday of Professor Fosong Wang (Part II) | Updated：2023-01-03

- CONTRIBUTION OF CHOLESTEROL MOIETIES ATTACHED ON MPEG-b-PCL-b-PLL TO THE CELL UPTAKE, ENDOSOMAL ESCAPE AND GENE KNOCKDOWN OF THE MICELLEPLEXES OF siRNA
- Chinese Journal of Polymer Science Vol. 31, Issue 6, Pages: 912-923(2013)
- Affiliations：
  
  a.State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  b.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  **Xia-bin Jing (景遐斌), E-mail: xbjing@ciac.jl.cn
- Funds：
- DOI：10.1007/s10118-013-1288-6
  CLC：
- Received：04 January 2013，
  
  Revised：2013-02-06，
  
  Accepted：18 February 2013，
  
  Published：2013-06
- Accepted：
Scan QR Code
Ruo-gu Qi, Su-hong Wu, Yu Wang, et al. CONTRIBUTION OF CHOLESTEROL MOIETIES ATTACHED ON MPEG-b-PCL-b-PLL TO THE CELL UPTAKE, ENDOSOMAL ESCAPE AND GENE KNOCKDOWN OF THE MICELLEPLEXES OF siRNA[J]. Chinese Journal of Polymer Science, 2013, 31(6): 912-923. DOI: 10.1007/s10118-013-1288-6.
DOI：

Ruo-gu Qi, Su-hong Wu, Yu Wang, et al. CONTRIBUTION OF CHOLESTEROL MOIETIES ATTACHED ON MPEG-b-PCL-b-PLL TO THE CELL UPTAKE, ENDOSOMAL ESCAPE AND GENE KNOCKDOWN OF THE MICELLEPLEXES OF siRNA[J]. Chinese Journal of Polymer Science, 2013, 31(6): 912-923. DOI: 10.1007/s10118-013-1288-6. DOI：

摘要

Abstract

To further enhance the transfection efficiency of a micelleplex system based on monomethoxy poly(ethylene glycol)-

block

-poly(

-caprolactone)-

block

-poly(L-lysine) (MPEG-

-PCL-

-PLL)

cholesterol (Chol) moieties are attached to the

-termini of PLL segments to obtain MPEG-

-PCL-

-PLL/Chol. The structure and morphology of the copolymer are studied by

H-NMR

TEM and DLS (dynamic light scattering). The cytotoxicity

cell uptake

endosomal release and mRNA knockdown are studied by MTT (3-[4

5-dimethylthiazol-2-yl]-2

5-diphenyltetrazolium bromide) assay

flow cytometry

CLSM (confocal laser scanning microscopy) and RT-PCR (real-time polymerase chain reaction). The results show that compared to their precursor MPEG-

-PCL-

-PLL

the cholesterol-grafted copolymer shows significantly lower toxicity

more rapid cellular endocytosis and endosome escape

and consequently displays enhanced siRNA transfection efficiency even at a lower N/P ratio. These improvements are ascribed to enhanced interaction of the cholesterol moieties with both cellular membrane and endosomal membrane. Moreover

effect of the PLL block length is examined. The final conclusion is that long enough PLL segments and incorporation of proper fraction of cholesterol onto the PLL segments benefit the enhancement of siRNA transfection efficiency.

关键词

Keywords

references

Views

229

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Construction of Magnetic Resonance Imaging Visible Polymeric Vector for Efficient Tumor Targeted siRNA Delivery

Amphiphilic Dendrimer-like Copolymers with High Chain Density by Living Anionic Polymerization

Multifunctional Cholesterol-modified Dendrimers for Targeted Drug Delivery to Cancer Cells Expressing Folate Receptors

Fabrication of Hydrophilic and Sponge-like PVDF/Brush-like Copolymer Blend Membranes Using Triethylphosphate as Solvent

Enhanced Segment Mobility Promotes Stereocomplex Crystallization in Polymer Blends via Elevated Segmental Miscibility

Related Author

Xin-Tao Shuai

Rong-Ze Wang

Si Huang

Qiao-Yun Zhang

Xing-Su Yu

Ke-Ze Hong

Jian-Rong Cao

Hong Xiao

Related Institution

College of Chemistry and Materials Science, Jinan University

Nanomedicine Research Center, The Third Affiliated Hospital, Sun Yat-sen University

Department of Reproductive Medical Center, Guangdong Women and Children Hospital

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University

Key Laboratory of Science & Technology of Eco-Textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University

⁰

CONTRIBUTION OF CHOLESTEROL MOIETIES ATTACHED ON MPEG-b-PCL-b-PLL TO THE CELL UPTAKE, ENDOSOMAL ESCAPE AND GENE KNOCKDOWN OF THE MICELLEPLEXES OF siRNA

DOI：10.1007/s10118-013-1288-6

摘要

Abstract

关键词

Keywords

references