A BIOREDUCIBLE POLYPEPTIDE FOR EFFICIENT GENE TRANSFECTION BOTH IN VITRO AND IN VIVO

Wei Qu; Si Chen; Shan Ren; Xue-jun Jiang; Ren-xi Zhuo; Xian-zheng Zhang

doi:10.1007/s10118-013-1270-3

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A BIOREDUCIBLE POLYPEPTIDE FOR EFFICIENT GENE TRANSFECTION BOTH IN VITRO AND IN VIVO

Special Issue: In Honor of the 80th Birthday of Professor Fosong Wang (Part I) | Updated：2023-01-03

- A BIOREDUCIBLE POLYPEPTIDE FOR EFFICIENT GENE TRANSFECTION BOTH IN VITRO AND IN VIVO
- Chinese Journal of Polymer Science Vol. 31, Issue 5, Pages: 713-718(2013)
- Affiliations：
  
  a.Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, China
  b.Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China
- Author bio：
  
  **Xian-zheng Zhang (张先正), E-mail: xz-zhang@whu.edu.cn
- Funds：
- DOI：10.1007/s10118-013-1270-3
  CLC：
- Received：18 December 2012，
  
  Revised：2013-01-18，
  
  Accepted：25 January 2013，
  
  Published：2013-05
- Accepted：
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Wei Qu, Si Chen, Shan Ren, et al. A BIOREDUCIBLE POLYPEPTIDE FOR EFFICIENT GENE TRANSFECTION BOTH IN VITRO AND IN VIVO[J]. Chinese Journal of Polymer Science, 2013, 31(5): 713-718.
DOI：

Wei Qu, Si Chen, Shan Ren, et al. A BIOREDUCIBLE POLYPEPTIDE FOR EFFICIENT GENE TRANSFECTION BOTH IN VITRO AND IN VIVO[J]. Chinese Journal of Polymer Science, 2013, 31(5): 713-718. DOI： 10.1007/s10118-013-1270-3.

摘要

Abstract

A disulfide bond containing polypeptide

PolyK

-R

was designed and prepared by oxidative polymerization between terminal cysteinyl thiol groups of CHK

HC and CR

C. The molar ratio between CHK

HC and CR

C within obtained PolyK

-R

was 9:1

and PolyK

-R

could combine DNA compactly when weight ratio reached 5. Through

in vitro

investigation

it was found that PolyK

-R

was an efficient gene vector with low cytotoxicity for delivering DNA in both COS-7 and HeLa cells. Cellular uptake of DNA mediated by PolyK

-R

/DNA complexes was promoted after incubation for 4 h. Moreover

by examining the histological sections of hindlimb ischemia rats through immunohistochemistry

PolyK

-R

/VEGF complexes were proved to be effective in both VEGF protein expression and succeeding vessel formation. The results indicated that polypeptide-based PolyK

-R

is a promising gene vector for the limb ischemia treatment.

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