ALTERED ENZYMATIC ACTIVITY OF LYSOZYMES BOUND TO VARIOUSLY SULFATED CHITOSANS

Hong-wei Wang; Lin Yuan; Tie-liang Zhao; He Huang; Hong Chen; Di Wu

doi:10.1007/s10118-012-1181-8

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ALTERED ENZYMATIC ACTIVITY OF LYSOZYMES BOUND TO VARIOUSLY SULFATED CHITOSANS

PAPER | Updated：2023-01-03

- ALTERED ENZYMATIC ACTIVITY OF LYSOZYMES BOUND TO VARIOUSLY SULFATED CHITOSANS
- Chinese Journal of Polymer Science Vol. 30, Issue 6, Pages: 893-899(2012)
- Affiliations：
  
  Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China
  Faculty of Applied Science & Engineering, University of Toronto, 35 St. George Street, Toronto, Ontario M5S 1A4, Canada
- Author bio：
  
  **Lin Yuan (袁琳), E-mail: yuanl@suda.edu.cn
  Hong Chen (陈红), E-mail: chenh@suda.edu.cn
- Funds：
- DOI：10.1007/s10118-012-1181-8
  CLC：
- Received：28 March 2012，
  
  Revised：2012-05-18，
  
  Accepted：25 May 2012，
  
  Published：2012-11
- Accepted：
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Hong-wei Wang, Lin Yuan, Tie-liang Zhao, et al. ALTERED ENZYMATIC ACTIVITY OF LYSOZYMES BOUND TO VARIOUSLY SULFATED CHITOSANS[J]. Chinese Journal of Polymer Science, 2012, 30(6): 893-899.
DOI：

Hong-wei Wang, Lin Yuan, Tie-liang Zhao, et al. ALTERED ENZYMATIC ACTIVITY OF LYSOZYMES BOUND TO VARIOUSLY SULFATED CHITOSANS[J]. Chinese Journal of Polymer Science, 2012, 30(6): 893-899. DOI： 10.1007/s10118-012-1181-8.

摘要

Abstract

The purpose of this research is to investigate the effects of the variously sulfated chitosans on lysozyme activity and structure. It was shown that the specific enzymatic activity of lysozyme remained almost similar to the native protein after being bound to 6-O-sulfated chitosan (6S-chitosan) and 3

6-O-sulfated chitosan (3

6S-chitosan)

but decreased greatly after being bound to 2-N-6-O-sulfated chitosan (2

6S-chitosan). Meanwhile

among these sulfated chitosans

6S-chitosan induced the greatest conformational change in lysozyme as indicated by the fluorescence spectra. These findings demonstrated that when sulfated chitosans of different structures bind to lysozyme

lysozyme undergoes conformational change of different magnitudes

which results in corresponding levels of lysozyme activity. Further study on the interaction of sulfated chitosans with lysozyme by surface plasmon resonance (SPR) suggested that their affinities might be determined by their molecular structures.

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