AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS

Xu-lin Jiang; Yan-feng Chu; Jia Liu; Guang-yan Zhang; Ren-xi Zhuo

doi:10.1007/s10118-011-1060-8

Your Location：

Home >

Browse articles >

AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS

Special Issue: The 8th International Symposium on Polyelectrolytes (ISP 2010) | Updated：2021-06-21

- AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS
- Chinese Journal of Polymer Science Vol. 29, Issue 4, Pages: 421-426(2011)
- Affiliations：
  
  Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, China
- Author bio：
  
  **Xu-lin Jiang (蒋序林), E-mail: xljiang@whu.edu.cn
- Funds：
- DOI：10.1007/s10118-011-1060-8
  CLC：
- Received：08 April 2011，
  
  Revised：2011-04-29，
  
  Accepted：03 May 2011，
  
  Published：2011
- Accepted：
Scan QR Code
Xu-lin Jiang, Yan-feng Chu, Jia Liu, et al. AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS[J]. Chinese Journal of Polymer Science, 2011, 29(4): 421-426.
DOI：

Xu-lin Jiang, Yan-feng Chu, Jia Liu, et al. AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS[J]. Chinese Journal of Polymer Science, 2011, 29(4): 421-426. DOI： 10.1007/s10118-011-1060-8.

摘要

Abstract

Cationic polymers have been receiving much attention as non-viral gene vectors. The aqueous mobile phase was optimized in combination with Shodex OHpak SB columns for size-exclusion chromatography (SEC) analysis of disulfide-containing poly(ethylene imine) (PEI) derivatives used in gene delivery. Addition of acetonitrile in mobile phase was shown to be able to suppress the hydrophobic interactions between polymer analytes and the stationary phase. The absolute molecular weights and distributions of the cationic polymers were determined directly from online SEC-MALS (multi-angle light scattering)/RI (refractive index detector). The results demonstrate that a good SEC separation of disulfide-containing PEI derivatives used in gene delivery with little band broadening was achieved.

关键词

Keywords

references

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers

The Effect of NMR Setting Parameters on Molecular Weight Determination of Polyether Diols

The New Methods for Characterization of Molecular Weight of Supramolecular Polymers

Durable Anti-fogging and Abrasion-resistant Coatings Based on Polyoxometalates-Hydrophilic Resin Composites with Enhanced Cohesion and Adhesion

Manipulating Hierarchical Aggregation Structure via Film-forming Kinetics Enables Organic Photovoltaics over 20% Efficiency

Related Author

Xiao-Ya Guo

Zhi-Yu Yang

Hua-Pan Fang

Dan-Hua Zhou

Xuan Pang

Hua-Yu Tian

Xue-Si Chen

Shu-Jie Huang

Related Institution

Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM)

State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University

School of Applied Chemistry and Engineering, University of Science and Technology of China

Changchun Institute of Applied Chemistry, Chinese Academy of Sciences

Jiangsu Engineering Laboratory of Novel Functional Polymeric Materials, State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University

⁰