SYNTHETIC HYDROGELS AS SCAFFOLDS FOR MANIPULATING ENDOTHELIUM CELL BEHAVIORS

Yong-mei Chen; Jing-jing Yang; Yoshihito Osada; Jian Ping Gong

doi:10.1007/s10118-010-1021-7

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SYNTHETIC HYDROGELS AS SCAFFOLDS FOR MANIPULATING ENDOTHELIUM CELL BEHAVIORS

Feature Article | Updated：2021-06-21

- SYNTHETIC HYDROGELS AS SCAFFOLDS FOR MANIPULATING ENDOTHELIUM CELL BEHAVIORS
- Chinese Journal of Polymer Science Vol. 29, Issue 1, Pages: 23-41(2011)
- Affiliations：
  
  a.MOE Key Laboratory for Non-equilibrium Condensed Matter and Quantum Engineering, Biomedical Engineering and Biomechanics Center, Department of Chemistry, School of Science, Xi'an Jiaotong University, Xi'an 710049, China
  b.Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan
  c.RIKEN 2-1, Hirosawa, Wako, Saitama 351-0198, Japan
- Author bio：
  
  *Yong-mei Chen (陈咏梅), E-mail: chenym@mail.xjtu.edu.cn
  Jian Ping Gong, E-mail: gong@sci.hokudai.ac.jp
- Funds：
- DOI：10.1007/s10118-010-1021-7
  CLC：
- Received：31 August 2010，
  
  Accepted：02 September 2010，
  
  Published：2011
- Accepted：
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Yong-mei Chen, Jing-jing Yang, Yoshihito Osada, et al. SYNTHETIC HYDROGELS AS SCAFFOLDS FOR MANIPULATING ENDOTHELIUM CELL BEHAVIORS[J]. Chinese Journal of Polymer Science, 2011, 29(1): 23-41.
DOI：

Yong-mei Chen, Jing-jing Yang, Yoshihito Osada, et al. SYNTHETIC HYDROGELS AS SCAFFOLDS FOR MANIPULATING ENDOTHELIUM CELL BEHAVIORS[J]. Chinese Journal of Polymer Science, 2011, 29(1): 23-41. DOI： 10.1007/s10118-010-1021-7.

摘要

Abstract

Synthetic hydrogels can be used as scaffolds that not only favor endothelial cells (ECs) proliferation but also manipulate the behaviors and functions of the ECs. In this review paper

the effect of chemical structure

Young's modulus (

) and zeta potential (

) of synthetic hydrogel scaffolds on static cell behaviors

including cell morphology

proliferation

cytoskeleton structure and focal adhesion

and on dynamic cell behaviors

including migration velocity and morphology oscillation

as well as on EC function such as anti-platelet adhesion

are reported. It was found that negatively charged hydrogels

poly(2-acrylamido-2-methylpropanesulfonic sodium) (PNaAMPS) and poly(sodium

-styrene sulphonate) (PNaSS)

can directly promote cell proliferation

with no need of surface modification by any cell-adhesive proteins or peptides at the environment of serum-containing medium. In addition

the Young's modulus (

) and zeta potential (

) of hydrogel scaffolds are quantitatively tuned by copolymer hydrogels

poly(NaAMPS-

-DMAAm) and poly(NaSS-

- DMAAm)

in which the two kinds of negatively charged monomers NaAMPS and NaSS are copolymerized with neutral monomer

-dimethylacrylamide (DMAAm). It was found that the critical zeta potential of hydrogels manipulating EC morphology

proliferation

and motility is

critical

= -20.83 mV and

critical

= -14.0 mV for poly(NaAMPS-

-DMAAm) and poly(NaSS-

-DMAAm)

respectively. The above mentioned EC behaviors well correlate with the adsorption of fibronectin

a kind of cell-adhesive protein

on the hydrogel surfaces. Furthermore

adhered platelets on the EC monolayers cultured on the hydrogel scaffolds obviously decreases with an increase of the Young's modulus (

) of the hydrogels

especially when

60 kPa. Glycocalyx assay and gene expression of ECs demonstrate that the anti-platelet adhesion well correlates with the EC-specific glycocalyx. The above investigation suggests that understanding the relationship between physic-chemical properties of synthetic hydrogels and cell responses is essential to design optimal soft and wet scaffolds for tissue engineering.

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