PROPERTIES AND SYNTHESIS OF NEW SUPPORTS FOR IMMOBILIZATION OF ENZYMES BY COPOLYMERIZATION OF VINYLENE CARBONATE AND METHACRYLIC ACID
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PROPERTIES AND SYNTHESIS OF NEW SUPPORTS FOR IMMOBILIZATION OF ENZYMES BY COPOLYMERIZATION OF VINYLENE CARBONATE AND METHACRYLIC ACID
Vol. 18, Issue 4, Pages: 343-349(2000)
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Published:20 August 2000
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PROPERTIES AND SYNTHESIS OF NEW SUPPORTS FOR IMMOBILIZATION OF ENZYMES BY COPOLYMERIZATION OF VINYLENE CARBONATE AND METHACRYLIC ACID[J]. 2000, 18(4): 343-349.
DOI:
PROPERTIES AND SYNTHESIS OF NEW SUPPORTS FOR IMMOBILIZATION OF ENZYMES BY COPOLYMERIZATION OF VINYLENE CARBONATE AND METHACRYLIC ACID[J]. 2000, 18(4): 343-349.DOI:
PROPERTIES AND SYNTHESIS OF NEW SUPPORTS FOR IMMOBILIZATION OF ENZYMES BY COPOLYMERIZATION OF VINYLENE CARBONATE AND METHACRYLIC ACID
Methacrylic acid first was neutralized with an aqueous solution of NaOH to pH=6.0~7.0
vinylene carbonate(VCA) was added to the solution
then monomers were copolymerized in paraffin oil by means of reverse-phase suspensionpolymerization and hydrophilic copolymeric supports were prepared. The properties of the supports were determined usingtrypsin and results show that the amount of enzymes coupled to the supports and the specific activity of immobilized trypsinare related to the content of VCA structure units
reaction time and concentration of enzyme solution
etc.
Abstract
Methacrylic acid first was neutralized with an aqueous solution of NaOH to pH=6.0~7.0
vinylene carbonate(VCA) was added to the solution
then monomers were copolymerized in paraffin oil by means of reverse-phase suspensionpolymerization and hydrophilic copolymeric supports were prepared. The properties of the supports were determined usingtrypsin and results show that the amount of enzymes coupled to the supports and the specific activity of immobilized trypsinare related to the content of VCA structure units
reaction time and concentration of enzyme solution
etc.
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