STUDY ON THE IMMOBILIZATION OF PAPAIN WITH A MACROPOROUS BEAD CARRIER OF COPOLYMER CONTAINING MONOMER UNITS OF NAMINOETHYL ACRYLAMIDE AND VINYL ALCOHOL*

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STUDY ON THE IMMOBILIZATION OF PAPAIN WITH A MACROPOROUS BEAD CARRIER OF COPOLYMER CONTAINING MONOMER UNITS OF NAMINOETHYL ACRYLAMIDE AND VINYL ALCOHOL*

Papers | Updated：2021-06-21

- STUDY ON THE IMMOBILIZATION OF PAPAIN WITH A MACROPOROUS BEAD CARRIER OF COPOLYMER CONTAINING MONOMER UNITS OF NAMINOETHYL ACRYLAMIDE AND VINYL ALCOHOL*
- Vol. 18, Issue 1, Pages: 25-31(2000)
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- Published：20 February 2000
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STUDY ON THE IMMOBILIZATION OF PAPAIN WITH A MACROPOROUS BEAD CARRIER OF COPOLYMER CONTAINING MONOMER UNITS OF NAMINOETHYL ACRYLAMIDE AND VINYL ALCOHOL*[J]. 2000, 18(1): 25-31.
DOI：

STUDY ON THE IMMOBILIZATION OF PAPAIN WITH A MACROPOROUS BEAD CARRIER OF COPOLYMER CONTAINING MONOMER UNITS OF NAMINOETHYL ACRYLAMIDE AND VINYL ALCOHOL*[J]. 2000, 18(1): 25-31. DOI：

摘要

A kind of macroporous bead carrier of copolymer containing monomer units of N-aminoethyl acrylamide and vinylalcohol was synthesized

i.e. the MR-AA carrier. Papain was immobilized on the carrier using glutaraldehyde as the couplingagent. The enzymatic activity of the immobilized papain was compared with free papain using casein as a substrate

and theeffects of glutaraldehyde concentration

temperature

time and papain amount added on the activity recovery were alsoinvestigated. The results show that the MR-AA carrier contains reactive primary amine groups

hydrophilic amido links and hydroxyl groups

as well as macroporous structures based on its matrix (MR-AV matrix)

furthermore

the activity recovery of papain in the immobilization could reach 48%～58%.In comparison with free papain

the resulting immobilized papain exhibits a remarkable thermostability and better reusability.

Abstract

A kind of macroporous bead carrier of copolymer containing monomer units of N-aminoethyl acrylamide and vinylalcohol was synthesized

and theeffects of glutaraldehyde concentration

temperature

time and papain amount added on the activity recovery were alsoinvestigated. The results show that the MR-AA carrier contains reactive primary amine groups

hydrophilic amido links and hydroxyl groups

as well as macroporous structures based on its matrix (MR-AV matrix)

furthermore

the activity recovery of papain in the immobilization could reach 48%～58%.In comparison with free papain

the resulting immobilized papain exhibits a remarkable thermostability and better reusability.

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