STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES

WANG Lingzhi; YUAN Hong; FANG Shibi; JIANG Yingyan

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STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES

Papers | Updated：2021-06-21

- STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES
- Vol. 11, Issue 4, Pages: 364-369(1993)
- Affiliations：
  
  1. Institute of Chemistry, Academia Sinica,Beijing,100080
  2. Academia Sinica
  3. Beijing 100080
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：20 December 1993
- Accepted：
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[1]王玲治,袁红,方世壁,江英彦.STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES[J].Chinese Journal of Polymer Science,1993,{4}(04):74-79.

WANG Lingzhi, YUAN Hong, FANG Shibi, et al. STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES[J]. 1993, 11(4): 364-369.
[1]王玲治,袁红,方世壁,江英彦.STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES[J].Chinese Journal of Polymer Science,1993,{4}(04):74-79. DOI：

WANG Lingzhi, YUAN Hong, FANG Shibi, et al. STUDIES ON IMMOBILIZED GLUCOSE OXIDASE BY DIETHYLAMINOETHYL CELLULOSE COMPLEXES[J]. 1993, 11(4): 364-369. DOI：

摘要

<正> The properties of immobilized glucose oxidase （GOD） by the complexes of diethylaminoethyl cellulose（DEAEC） with different polymers

such as polymethylacrylic acid （PMAA）

polyacrylic acid （PAA）

polystyrene sulfonic acid （PSSA）

polyvinylaleohol （PVA）

polyethylene oxide （PEO） and styrene-maleic acid copolymer （PSMA） were investigated. The activity of immobilized GOD was obviously influenced by the component of the DEAEC complexes. The relative activity of the immobilized GOD reached to maximum and over 90% of the native GOD. when the DEAEC-PMAA DEAEC-PAA complexes were used as a carrier with the molar ratio of DEAEC and polyacid of about one. Michaelis constants （Km） of the immobilized enzymes of DEAEC-GOD-PMAA and DEAEC-GOD-PAA were determined to be 1.25 and 1.00

respectively. Moreover

the immobilized GOD has a good storage stability and cyclic life.

Abstract

The properties of immobilized glucose oxidase （GOD） by the complexes of diethylaminoethyl cellulose（DEAEC） with different polymers

such as polymethylacrylic acid （PMAA）

polyacrylic acid （PAA）

polystyrene sulfonic acid （PSSA）

polyvinylaleohol （PVA）

respectively. Moreover

the immobilized GOD has a good storage stability and cyclic life.

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