Dendritic poly(amidoamine)-b-poly(L-glutamate) (PAMAM-b-PLG) biohybrids were synthesized by the ring-opening polymerization of γ-benzyl-L-glutamate N-carboxyanhydride monomer

followed by the deprotection of benzyl groups on poly(benzyl-L-glutamate)

and were characterized by 1H-NMR

FT-IR and gel permeation chromatography. The self-assembly behavior of the PAMAM-b-PLG biohybrid was investigated by means of UV-Vis

dynamic light scattering (DLS)

transmission electronic microscopy (TEM) and 1H-NMR. UV-Vis analysis demonstrated that the critical aggregation concentration of PAMAM-b-PLG was dependent on the pH value of aqueous solutios. DLS results further evidenced that the PAMAM-b-PLG biohybrid exhibited a pH-sensitive self-assembly behavior

and the average size of the self-assembled nanoparticles decreased gradually with the increasing pH value of the PAMAM-b-PLG solution. The self-assembled nanoparticles gave a nearly spherical morphology

and the pH-induced self-assembly had no obvious effect on the morphology of nanoparticles.