Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging

Shi-Lu Ji; Hua-Min Lan; Sen-Sen Zhou; Xiao-Ke Zhang; Wei-Zhi Chen; Xi-Qun Jiang

doi:10.1007/s10118-023-2922-6

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Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging

RESEARCH ARTICLE | Updated：2023-04-21

- Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging
- Chinese Journal of Polymer Science Vol. 41, Issue 5, Pages: 794-801(2023)
- Affiliations：
  
  a.Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China
  b.College of Science, Nanjing Forestry University, Nanjing 210037, China
- Author bio：
  
  chenwz@nju.edu.cn(W.Z.C)
  jiangx@nju.edu.cn(X.Q.J)
- Funds：
- DOI：10.1007/s10118-023-2922-6
  CLC：
- Received：26 October 2022，
  
  Revised：2022-12-5，
  
  Accepted：06 December 2022，
  
  Online First：16 January 2023，
  
  Published：01 May 2023
- Accepted：
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Ji, S. L.; Lan, H. M.; Zhou, S. S.; Zhang, X. K.; Chen, W. Z.; Jiang, X. Q. Ir(III)-based ratiometric hypoxic probe for cell imaging.Chinese J. Polym. Sci.2023,41, 794–801

Shi-Lu Ji, Hua-Min Lan, Sen-Sen Zhou, et al. Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging[J]. Chinese Journal of Polymer Science, 2023, 41(5): 794-801.
Ji, S. L.; Lan, H. M.; Zhou, S. S.; Zhang, X. K.; Chen, W. Z.; Jiang, X. Q. Ir(III)-based ratiometric hypoxic probe for cell imaging.Chinese J. Polym. Sci.2023,41, 794–801 DOI： 10.1007/s10118-023-2922-6.

Shi-Lu Ji, Hua-Min Lan, Sen-Sen Zhou, et al. Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging[J]. Chinese Journal of Polymer Science, 2023, 41(5): 794-801. DOI： 10.1007/s10118-023-2922-6.

摘要

Two new ratiometric hypoxia probes (Ir-C343 and Ir-GFP) are synthesized

and a ratiometric response is realized that is only related to oxygen concentration. Ir-GFP shows promising applications in the ratiometric hypoxia imaging of cells due to its long excitation wavelength

good water solubility

high biocompatibility

and low relative fluorescence intensity compared with the phosphorescent emitter Ir-fliq.

Abstract

Two new ratiometric hypoxia probes (Ir-C343 and Ir-GFP) are synthesized by covalently incorporating florescent internal standard molecules coumarin 343 (C343) and green fluorescent protein (GFP) into bis[1-(9

9-dimethyl-9

-fluoren-2-yl)-isoquinoline] (succinylacetone) Ir(III) (Ir-fliq)

respectively. After connecting with internal standard molecules

the Ir-fliq moiety still exhibits high sensitivity to oxygen concentration

while the fluorescence intensity of the internal standard remains relatively constant under different oxygen concentrations. As a result

a ratiometric response is realized that is only related to oxygen concentration. In addition

Ir-GFP shows more promising applications in the ratiometric hypoxia imaging of cells due to its long excitation wavelength

good water solubility

high biocompatibility

and low relative fluorescence intensity compared with the phosphorescent emitter Ir-fliq.

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references

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